OSTRC questionnaire on health problems has been translated to Norwegian, Danish and Swedish. Due to the lack of similar questionnaires in Iran and the necessity to use innovative and reliable tools in sport epidemiology, localization and investigation of validity and reliability of the Persian version of this questionnaire was the aim of this study. The necessary modifications were conducted and the final form of the questionnaires was prepared using translation-back-translation method. CVR and CVI methods were used to determine content validity, internal consistency (Cronbach alpha) was used to determine reliability and test-retest method was used to evaluate reliability. Kappa agreement coefficient non-parametric test was also used to assess the reliability of other items of the questionnaire. CVI results showed that all items had CVI scores greater than 0.79 (relevance 0.92, clarity 0.98, simplicity 0.99 and ambiguity 0.94). S-CVI/Ave of the questionnaire was 0.95 (0.85-1). Statistical analysis showed that first four items had internal consistency of 0.89. Kappa agreement coefficient was 0.71 to 0.81 (P=0.001). Finally, according to these findings, OSTRC questionnaire on health problems showed the emergence of a new method to record a variety of health problems in sport. So this method is reliable and valid when monitoring and recording acute injuries, chronic injuries as well as diseases in athlete population.